jf646 mbp (Novus Biologicals)

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Novus Biologicals jf646 mbp
Jf646 Mbp, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/jf646+mbp/MBP+Antibody+(2H9)+%5BJanelia+Fluor%C2%AE+646%5D/pm40537468-396-106-107
Average 93 stars, based on 3 article reviews

jf646 mbp - by Bioz Stars, 2026-09

93/100 stars

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Article Title: TGFα controls checkpoints in CNS resident and infiltrating immune cells to promote resolution of inflammation
Article Snippet: The following antibodies were used in the study: BV421-CD11b (Biolegend, #101235), BV480-CD11c (BD, #565627), BV510-F4/80 (Biolegend, #123135), BV570-Ly6C (Biolegend, #128029), BV605-CD80 (BD, #563052), BV650-CD56 (BD, #748098), BV650-CD8 (BD, #100741), PE-eFlour610-CD140a (Thermo Fisher Scientific, #61140180), SuperBright780-MHCII (Thermo Fisher Scientific, #78532080), BV711-CD74 (BD, #740748), AF488-EGFR (Cell Signaling, #5616), PE-Cy7-Tmem119 (Thermo Fisher Scientific, #25611980), eFluor 450-CD3 (Thermo Fisher Scientific, #48003742), BV605-CD68 (Biolegend, #137021), BV711-CD74 (BD, #740748), SB600-CD140B (Thermo Fisher Scientific, #63140282), PE-CD105 (Thermo Fisher Scientific, #12-1051-82), AF488-A2B5 (Novus Biologicals, #FAB1416G), PE-Cy5-CD24 (Biolegend, #101811), PE-Cy7-CD31 (Thermo Fisher Scientific, #25031182), PerCP-eFlour710-CD86 (Thermo Fisher Scientific, #46086280), AF532-CD44 (Thermo Fisher Scientific, #58044182), PE-B220 (BD, #561878), PE-ACSA1 (Miltenyi Biotech, #130118483), PE-Cy5.5-CD45 (Thermo Fisher Scientific, #35045180), JF646-MBP (Novus Biologicals, #NBP2-22121JF646), APC-Cy7-Ly6G (Biolegend, #127623), AF700-O4 (R&D, #FAB1326N), BUV737-CD154 (BD, #741735), AF660-CD19 (Thermo Fisher Scientific, #606019380), APC/Fire810-CD4 (Biolegend, #100479), PE-ACSA2 (Miltenyi Biotech, #130117386).

Article Title: PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation
Article Snippet: Antibodies used in this study were: BV421-CD11b (1:200; Biolegend, #101235), BV480-CD11c (1:100; BD, #565627), BV510-F4/80 (1:100; Biolegend, #123135), BV570-Ly6C (1:200; Biolegend, #128029), BV605-CD80 (1:100; BD, #563052), BV650-CD56 (1:100; BD, #748098), BV650-CD8 (1:100; Biolegend, #100741), PE-eFlour610-CD140a (1:100; Thermo Fisher Scientific, #61140180), SuperBright780-MHCII (1:200; Thermo Fisher Scientific, #78532080), BV711-CD74 (1:200; BD, #740748), PE-CD45R/B220 (1:100; BD, #561878), PE-CD105 (1:100; Thermo Fisher Scientific, #12-1051-82), PE-Ly6G (1:200; BioLegend, #127607), PE-CD140a (1:100; BioLegend, #135905), PE-O4 (1:100; Miltenyi, #130117507), PE-Ter119 (1:100; Biolegend, #116207), PE-Ly6C (1:100; Biolegend, #128007), AF488-A2B5 (1:100; Novus Biologicals, #FAB1416G), PE-CD279 (1:100; Biolegend, #135206), PE-CD273 (1:100; Biolegend, #107205), AF488-CD274 (1:100; Thermo Fisher Scientific, #53598282), PE-Cy5-CD24 (1:200; Biolegend, #101811), PE-Cy7-CD31 (1:200; Thermo Fisher Scientific, #25031182), PE-Cy7-CD274 (1:100; Thermo Fisher Scientific, #12598282), PerCP-eFlour710-CD86 (1:100; Thermo Fisher Scientific, #46086280), AF532-CD44 (1:100; Thermo Fisher Scientific, #58044182), PE-Cy5.5-CD45 (1:200; Thermo Fisher Scientific, #35045180), JF646-MBP (1:100; Novus Biologicals, #NBP2-22121JF646), APC-Cy7-Ly6G (1:200; Biolegend, #127623), AF700-O4 (1:200; R&D, #FAB1326N), BUV737-CD154 (1:100; BD, #741735), AF660-CD19 (1:100; Thermo Fisher Scientific, #606019380), APC/Fire810-CD4 (1:100; Biolegend, #100479).

Article Title: The astrocyte-produced growth factor HB-EGF limits autoimmune CNS pathology
Article Snippet: Antibodies used in this study were: BV421-CD11b (Biolegend, no. 101235; 1:200), BV480-CD11c (BD, no. 565627, 1:100), BV510-F4/80 (Biolegend, no. 123135, 1:100), BV570-Ly6C (Biolegend, no. 128029, 1:200), BV605-CD80 (BD, no. 563052, 1:100), BV650-CD56 (BD, no. 748098, 1:100), BV650-CD8 (BD, no. 100741, 1:100), PE-eFlour610-CD140a (Thermo Fisher Scientific, no. 61140180, 1:100), SuperBright780-MHCII (Thermo Fisher Scientific, no. 78532080, 1:200), BV711-CD74 (BD, no. 740748, 1:200), PE-CD45R/B220 (BD, no. 561878, 1:100), PE-CD105 (Thermo Fisher Scientific, no. 12-1051-82, 1:100), PE-Ly6G (BioLegend, no. 127607, 1:200), PE-CD140a (BioLegend, no. 135905, 1:100), PE-O4 (Miltenyi, no. 130117507, 1:100), PE-Ter119 (Biolegend, no. 116207), PE-Ly6C (Biolegend, no. 128007, 1:100), AF488-A2B5 (Novus Biologicals, no. FAB1416G, 1:100), PE-Cy5-CD24 (Biolegend, no. 101811, 1:200), PE-Cy7-CD31 (Thermo Fisher Scientific, no. 25031182, 1:200), PerCP-eFlour710-CD86 (Thermo Fisher Scientific, no. 46086280, 1:100), AF532-CD44 (Thermo Fisher Scientific, no. 58044182, 1:100), PE-Cy5.5-CD45 (Thermo Fisher Scientific, no. 35045180, 1:300), JF646-MBP (Novus Biologicals, no. NBP2-22121JF646, 1:100), APC-Cy7-HB-EGF (Bioss, no. BS-3576R-APC-CY7, 1:100), APC-Cy7-Ly6G (Biolegend, no. 127623, 1:200), AF700-O4 (R&D, no. FAB1326N, 1:200, 1:100), BUV737-CD154 (BD, no. 741735, 1:100), AF660-CD19 (Thermo Fisher Scientific, no. 606019380, 1:100), APC/Fire810-CD4 (Biolegend, no. 100479, 1:100).

Article Title: Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation
Article Snippet: Antibodies used in this study were: BV421-CD11b (Biolegend, #101235), eF450-CD3 (Thermo Fisher Scientific Scientific, # 48-0031-80), BV480-CD11c (BD, #565627), BV510-F4/80 (Biolegend,# 123135), BV570-Ly6C (Biolegend,# 128029), BV605-CD80 (BD, #563052), BV650-CD56 (BD, #748098), BV650-CD8 (Biolegend, #100741), PE-eFlour610-CD140a (Thermo Fisher Scientific Scientific, #61140180), SuperBright780-MHCII (Thermo Fisher Scientific Scientific, #78532080), BV711-CD74 (BD, #740748), PE-B220 (BD, #561878), PE-CD105 (Thermo Fisher Scientific Scientific, # 12-1051-82), AF488-A2B5 (Novus Biologicals, #FAB1416G), PE-Cy5-CD24 (Biolegend, #101811), PerCP-eFlour710-CD86 (Thermo Fisher Scientific Scientific, #46086280), AF532-CD44 (Thermo Fisher Scientific Scientific, #58044182), PE-Cy5.5-CD45 (Thermo Fisher Scientific Scientific, #35045180), JF646-MBP (Novus Biologicals, #NBP2-22121JF646), APC-Cy7-Ly6G (Biolegend, #127623), AF700-O4 (R&D, #FAB1326N), BUV737-CD154 (BD, #741735), AF660-CD19 (Thermo Fisher Scientific Scientific, #606019380), APC/Fire810-CD4 (Biolegend, #100479).

Article Title: The astrocyte-produced growth factor HB-EGF limits autoimmune CNS pathology.
Article Snippet: For intracellular flow cytometry staining, cells were fixed overnight after surface staining using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (eBioscience, no. 00552300) according to the manufacturer’s instructions.

Article Title: TGFα controls checkpoints in CNS resident and infiltrating immune cells to promote resolution of inflammation.
Article Snippet: The following antibodieswere used in the study: BV421-CD11b (Biolegend, #101235), BV480-CD11c (BD, #565627), BV510-F4/80 (Biolegend, #123135), BV570-Ly6C (Biolegend, #128029), BV605-CD80 (BD, #563052), BV650-CD56 (BD, #748098), BV650-CD8 (BD, #100741), PE-eFlour610-CD140a (Thermo Fisher Scientific, #61140180), SuperBright780-MHCII (Thermo Fisher Scientific, #78532080), BV711-CD74 (BD, #740748), AF488-EGFR (Cell Signaling, #5616), PE-Cy7-Tmem119 (Thermo Fisher Scientific, #25611980), eFluor 450-CD3 (Thermo Fisher Scientific, #48003742), BV605-CD68 (Biolegend, #137021), BV711-CD74 (BD, #740748), SB600-CD140B (Thermo Fisher Scientific, #63140282), PE-CD105 (Thermo Fisher Scientific, #12-1051-82), AF488-A2B5 (Novus Biologicals, #FAB1416G), PECy5-CD24 (Biolegend, #101811), PE-Cy7-CD31 (Thermo Fisher Scientific, #25031182), PerCP-eFlour710-CD86 (Thermo Fisher Scientific, #46086280), AF532-CD44 (Thermo Fisher Scientific, #58044182), PEB220 (BD, #561878), PE-ACSA1 (Miltenyi Biotech, #130118483), PECy5.5-CD45 (Thermo Fisher Scientific, #35045180), JF646-MBP (Novus Biologicals, #NBP2-22121JF646), APC-Cy7-Ly6G (Biolegend, #127623), AF700-O4 (R&D, #FAB1326N), BUV737-CD154 (BD, #741735), AF660CD19 (Thermo Fisher Scientific, #606019380), APC/Fire810-CD4 (Biolegend, #100479), PE-ACSA2 (Miltenyi Biotech, #130117386).

Article Title: PD-L1 positive astrocytes attenuate inflammatory functions of PD-1 positive microglia in models of autoimmune neuroinflammation.
Article Snippet: Antibodies used in this study were: BV421-CD11b (1:200; Biolegend, #101235), BV480-CD11c (1:100; BD, #565627), BV510-F4/80 (1:100; Biolegend, #123135), BV570-Ly6C (1:200; Biolegend, #128029), BV605-CD80 (1:100; BD, #563052), BV650-CD56 (1:100; BD, #748098), BV650-CD8 (1:100; Biolegend, #100741), PE-eFlour610-CD140a (1:100; Thermo Fisher Scientific, #61140180), SuperBright780-MHCII (1:200; Thermo Fisher Scientific, #78532080), BV711-CD74 (1:200; BD, #740748), PE-CD45R/B220 (1:100; BD, #561878), PE-CD105 (1:100; Thermo Fisher Scientific, #12-1051-82), PE-Ly6G (1:200; BioLegend, #127607), PE-CD140a (1:100; BioLegend, #135905), PE-O4 (1:100; Miltenyi, #130117507), PE-Ter119 (1:100; Biolegend, #116207), PE-Ly6C (1:100; Biolegend, #128007), AF488-A2B5 (1:100; Novus Biologicals, #FAB1416G), PE-CD279 (1:100; Biolegend, #135206), PE-CD273 (1:100; Biolegend, #107205), AF488-CD274 (1:100; Thermo Fisher Scientific, #53598282), PE-Cy5-CD24 (1:200; Biolegend, #101811), PE-Cy7-CD31 (1:200; Thermo Fisher Scientific, #25031182), PE-Cy7-CD274 (1:100; Thermo Fisher Scientific, #12598282), PerCP-eFlour710-CD86 (1:100; Thermo Fisher Scientific, #46086280), AF532-CD44 (1:100; Thermo Fisher Scientific, #58044182), PE-Cy5.5-CD45 (1:200; Thermo Fisher Scientific, #35045180), JF646-MBP (1:100; Novus Biologicals, #NBP222121JF646), APC-Cy7-Ly6G (1:200; Biolegend, #127623), AF700-O4 (1:200; R&D, #FAB1326N), BUV737-CD154 (1:100; BD, #741735), AF660CD19 (1:100; ThermoFisher Scientific,#606019380), APC/Fire810-CD4 (1:100; Biolegend, #100479).

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